Journal: Bio-protocol

Article Title: Rapid Detection of Proliferative Bacteria by Electrical Stimulation

doi: 10.21769/BioProtoc.3508

Figure Lengend Snippet: List of equipment for Example Essential Equipment

Article Snippet: Multi-stage Fluorescence Microscope , Leica DMi8 microscope with Leica CTR advanced electronics box (Leica).

Techniques: Fluorescence, Microscopy, Incubation

Journal: Food Chemistry: X

Article Title: ‘Fengtang’ plum seed waste: Phytochemicals and anti-inflammatory effects in vivo and in vitro

doi: 10.1016/j.fochx.2026.103793

Figure Lengend Snippet: The impact of ‘Fengtang’ plum seed WE and EE on inflammatory factor release in RAW264.7 macrophage with LPS stimulation and the influence of ‘Fengtang’ plum seed EE on LPS-stimulated cell morphology and ROS level. (A) WE and EE's cytotoxic effects on RAW264.7 macrophages. (B) NO kit tested the release of NO. (C—F) ELISA kits detected PGE 2 (C), IL-1β (D), IL-6 (E), and TNF-α (F) contents in cell supernatants. (G) The morphology of macrophages was observed using an inverted microscope. (H) After staining with the DCFH-DA fluorescent probe, intracellular ROS was examined using fluorescence microscopy. In the cytotoxicity assay, the asterisk is used to indicate significant differences versus untreated cells (*** p < 0.001). In the detection of inflammatory factor release, significant differences are denoted by different letters ( p < 0.05).

Article Snippet: We acquired the ELISA kits for mouse TNF-α and IL-6 from Lianke Biotech, Co., Ltd. (Hangzhou, China).

Techniques: Enzyme-linked Immunosorbent Assay, Inverted Microscopy, Staining, Fluorescence, Microscopy, Cytotoxicity Assay

Journal: Food Chemistry: X

Article Title: ‘Fengtang’ plum seed waste: Phytochemicals and anti-inflammatory effects in vivo and in vitro

doi: 10.1016/j.fochx.2026.103793

Figure Lengend Snippet: Effects of ‘Fengtang’ plum seed EE on LPS-stimulated iNOS and COX-2 expression as well as inflammatory cytokine mRNA expression. (A, B) Western blot detection of the iNOS (A) and COX-2 (B) protein levels. (C-G) qRT-PCR determination of iNOS (C), COX-2 (D), IL-1β (E), IL-6 (F), and TNF-α (G) mRNA expression levels. GAPDH served as the internal control. In comparison to the control group, ### p < 0.001. *** p < 0.001 vs. LPS group.

Article Snippet: We acquired the ELISA kits for mouse TNF-α and IL-6 from Lianke Biotech, Co., Ltd. (Hangzhou, China).

Techniques: Expressing, Western Blot, Quantitative RT-PCR, Control, Comparison

Journal: Food Chemistry: X

Article Title: ‘Fengtang’ plum seed waste: Phytochemicals and anti-inflammatory effects in vivo and in vitro

doi: 10.1016/j.fochx.2026.103793

Figure Lengend Snippet: Effects of ‘Fengtang’ plum seed EE on the inflammatory cytokine levels and oxidative stress indices in tissue fluid and serum of mice induced by DSS. (A, B) Effects of ‘Fengtang’ plum seed EE on IL-1β, IL-6, and TNF-α concentrations in mouse tissue fluid and serum. (C, D) Effects of ‘Fengtang’ plum seed EE on the MDA content and enzymatic activities of CAT and SOD in mouse tissue fluid and serum. Substantial variances are indicated by distinct letters on the bars ( p < 0.05).

Article Snippet: We acquired the ELISA kits for mouse TNF-α and IL-6 from Lianke Biotech, Co., Ltd. (Hangzhou, China).

Techniques:

Journal: Antioxidants

Article Title: Isorhamnetin Attenuates Isoproterenol-Induced Myocardial Injury by Reducing ENO1 (Alpha-Enolase) in Cardiomyocytes

doi: 10.3390/antiox14050579

Figure Lengend Snippet: Isorhamnetin ameliorates ISO-induced apoptosis in rat heart tissue. ( A ) Effect of isorhamnetin on ISO-induced myocardial apoptosis, as shown by TUNEL staining. ( B ) Statistical analysis of TUNEL assay results for each group, n = 6. ( C , D ) Effects of isorhamnetin on ISO-induced expression of Bcl-2, Bax, and cleaved caspase-3 proteins in the rat myocardium, n = 3. Data are expressed as means ± SDs. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. the control group; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. the ISO group.

Article Snippet: Apoptosis was assessed using the Annexin V-FITC/PI Apoptosis Detection Kit (Multi Sciences, Hangzhou, China).

Techniques: TUNEL Assay, Staining, Expressing, Control

Journal: Antioxidants

Article Title: Isorhamnetin Attenuates Isoproterenol-Induced Myocardial Injury by Reducing ENO1 (Alpha-Enolase) in Cardiomyocytes

doi: 10.3390/antiox14050579

Figure Lengend Snippet: Isorhamnetin ameliorates ISO-induced H9c2 cardiomyocyte injury. ( A ) Effect of ISO on the survival rate of H9c2 cardiomyocytes, n = 6. ( B ) Effects of different concentrations of isorhamnetin on the survival rate of H9c2 cells, n = 6. ( C ) Effect of isorhamnetin on the survival rate of ISO-treated H9c2 cells, n = 6. ( D ) Effect of isorhamnetin on LDH activity in ISO-treated H9c2 cells, n = 6. ( E , F ) Effects of isorhamnetin on ISO-induced apoptosis in H9c2 cells, assessed by flow cytometry, n = 5. ( G , H ) JC-1 staining combined with flow cytometry analysis, n = 3. ( I , J ) JC-1 staining observed under an inverted microscope, n = 6. ( K , L ) Effects of isorhamnetin on ISO-induced protein expression of Bax, Bcl-2, and cleaved caspase-3 in H9c2 cells, as detected via Western blotting, n = 3. Data are expressed as means ± SDs. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. the control group; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. the ISO group.

Article Snippet: Apoptosis was assessed using the Annexin V-FITC/PI Apoptosis Detection Kit (Multi Sciences, Hangzhou, China).

Techniques: Activity Assay, Flow Cytometry, Staining, Inverted Microscopy, Expressing, Western Blot, Control

Journal: Antioxidants

Article Title: Isorhamnetin Attenuates Isoproterenol-Induced Myocardial Injury by Reducing ENO1 (Alpha-Enolase) in Cardiomyocytes

doi: 10.3390/antiox14050579

Figure Lengend Snippet: Isorhamnetin inhibits ENO1, activates the PPARα/PGC-1α signaling axis, reverses the transformation of H9c2 energy metabolism substrates induced by isoproterenol, inhibits glycolysis, increases ATP release, alleviates apoptosis and oxidative stress, and protects against cardiac injury. ( A – C ) Statistical graphs showing lactate, glucose, and ATP contents, n = 5, 6. ( D , E ) Western blot analysis of apoptosis, oxidative stress, and PPAR-signaling-pathway-related protein expression. β-actin was used as a control, n = 3. Data are expressed as means ± SDs. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. the control group; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. the ISO group.

Article Snippet: Apoptosis was assessed using the Annexin V-FITC/PI Apoptosis Detection Kit (Multi Sciences, Hangzhou, China).

Techniques: Transformation Assay, Western Blot, Expressing, Control

Journal: Advanced Science

Article Title: Circulating Tumor Cells Shed Shearosome Extracellular Vesicles in Capillary Bifurcations That Activate Endothelial and Immune Cells

doi: 10.1002/advs.202506339

Figure Lengend Snippet: HUVECs, THP‐1 monocytes, and THP‐1 differentiated M1 macrophages internalize LEVs. A–C) Orthogonal cross‐sectioning of multifluorescent confocal images validating the internalization of LEVs by HUVECs (A), monocytes (B), and M1 macrophages (C). Cells were stained with CMTPX cell tracker (shown as magenta) and LEVs with CMFDA cell tracker (green). Scale bar 20 µm. Arrows indicate the internalized LEV in all planes. D–F) Quantification of LEVs internalization frequency (double positive events) by HUVECs (D), monocytes (E), and M1 macrophages (F) ( n = 3 at least). All data are presented as mean ± standard deviation (SD). P ‐values annotated as: * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001, or not significant (ns). Two‐tailed Student's t ‐test was performed for all analyses.

Article Snippet: Where required, cell transit through the microfluidic devices ( n = 3 per geometry) was imaged using Nikon Eclipse Ti2 multi‐fluorescent inverted microscope (Nikon, UK) with Okolab incubated stage (Nikon, UK).

Techniques: Staining, Standard Deviation, Two Tailed Test

Journal: Advanced Science

Article Title: Circulating Tumor Cells Shed Shearosome Extracellular Vesicles in Capillary Bifurcations That Activate Endothelial and Immune Cells

doi: 10.1002/advs.202506339

Figure Lengend Snippet: LEVs polarize monocytes and M1 macrophages to CD206 + M2 tumor‐promoting macrophages. A) Percentage of monocytes that remained adhered. Monocytes were co‐cultured with LEVs or cultured with CM from LEVs or CM from MDA‐MB231 cells. Untreated monocytes served as a negative control. ( n = 3). One‐way ANOVA. B) Multifluorescent images of untreated or LEV‐treated monocytes stained for CD206 (red) and TNF‐a (green). Scale bar 50 µm. C,D) Histogram (C) and quantification (D) of CD206 mean fluorescence intensity for untreated monocytes, LEV‐treated monocytes, or monocytes treated with LEVs’ CM for 30 h, analyzed via flow cytometry ( n = 3). One‐way ANOVA. E,F) Dot plots (E) and quantification (F) of CD206‐positive events for untreated monocytes (left), LEV‐treated monocytes (middle), monocytes treated with LEVs’ CM (right) and monocytes treated with LEVs from siTGFβ‐treated MDA‐MB‐231 cells for 30 h, analyzed via flow cytometry ( n = 3). One‐way ANOVA. G,H) Reverse transcription quantitative polymerase chain reaction of untreated or LEV‐treated cells for genes CD206 (left), Arginase‐1 (Arg‐1) (middle) and fibronectin (FN) (right), for monocytes (G) and M1 macrophages (H) ( n = 3). Two‐tailed Student's t‐ test. All data are presented as mean ± standard deviation (SD). P values annotated as: * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001, or not significant (ns).

Article Snippet: Where required, cell transit through the microfluidic devices ( n = 3 per geometry) was imaged using Nikon Eclipse Ti2 multi‐fluorescent inverted microscope (Nikon, UK) with Okolab incubated stage (Nikon, UK).

Techniques: Cell Culture, Negative Control, Staining, Fluorescence, Flow Cytometry, Reverse Transcription, Real-time Polymerase Chain Reaction, Two Tailed Test, Standard Deviation